CIPSM - Research Area C

Research Area C

Fusion of Bacterial Flagellin to a Dendritic Cell-Targeting αCD40 Antibody Construct Coupled With Viral or Leukemia-Specific Antigens Enhances Dendritic Cell Maturation and Activates Peptide-Responsive T Cells

Cathepsin S Alterations Induce a Tumor-Promoting Immune Microenvironment in Follicular Lymphoma

2019

TLR8 Is a Sensor of RNase T2 Degradation Products

Challenges and perspectives for structural biology of lncRNAs—the example of the Xist lncRNA A-repeats

KMT9 monomethylates histone H4 lysine 12 and controls proliferation of prostate cancer cells

2018

The Antibody Light-Chain Linker Regulates Domain Orientation and Amyloidogenicity

A single residue switch reveals principles of antibody domain integrity

Targeting TRAF6 E3 ligase activity with a small molecule inhibitor combats autoimmunity

Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1

Protons as Versatile Reporters in Solid-State NMR Spectroscopy

A switch point in the molecular chaperone Hsp90 responding to client interaction

Von einer Helix zu einem kleinen Ring: Metadynamik‐inspirierte, selektive Liganden für αvβ6‐Integrin

Structural basis for ATP-dependent chromatin remodelling by the INO80 complex

In vitro iCLIP-based modeling uncovers how the splicing factor U2AF2 relies on regulation by cofactors

Development of Novel Melanocortin Receptor Agonists Based on the Cyclic Peptide Framework of Sunflower Trypsin Inhibitor-1

Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments

N-Methylation of isoDGR Peptides: Discovery of a Selective α5β1-Integrin Ligand as a Potent Tumor Imaging Agent

Therapeutic Radiopharmaceuticals Targeting Integrin αvβ6

Dynamics and Interactions of a 29 kDa Human Enzyme Studied by Solid-State NMR

2017

A Numb–Mdm2 fuzzy complex reveals an isoform-specific involvement of Numb in breast cancer

Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands

Microsecond Timescale Protein Dynamics: a Combined Solid‐State NMR Approach

Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference

The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3

cGAS senses long and HMGB/TFAM-bound U-turn DNA by forming protein–DNA ladders

Structural insights into the interaction of the nuclear exosome helicase Mtr4 with the preribosomal protein Nop53

Exploring the Role of RGD-Recognizing Integrins in Cancer

Improving oral bioavailability of cyclic peptides by N-methylation

Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions

A chemical compound inhibiting the Aha1-Hsp90 chaperone complex

Genetic regulatory effects modified by immune activation contribute to autoimmune disease associations

Expression and purification of a difficult sarcomeric protein: Telethonin

Effects of Fluorophore Attachment on Protein Conformation and Dynamics Studied by spFRET and NMR Spectroscopy

MAK33 antibody light chain amyloid fibrils are similar to oligomeric precursors

Structural Insights into Selective Ligand–Receptor Interactions Leading to Receptor Inactivation Utilizing Selective Melanocortin 3 Receptor Antagonists

RNA structure refinement using NMR solvent accessibility data

Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins

Inhibitors of glycosomal protein import provide new leads against trypanosomiasis

Fibronectin promotes directional persistence in fibroblast migration through interactions with both its cell-binding and heparin-binding domains

Microsecond Time Scale Proton Rotating-Frame Relaxation under Magic Angle Spinning

Structure-function analysis of the DNA-binding domain of a transmembrane transcriptional activator

Discrimination of Cytosolic Self and Non-Self RNA by RIG-I-like Receptors

Protein conformational dynamics studied by 15N and 1H R1ρ relaxation dispersion: Application to wild-type and G53A ubiquitin crystals

Non-equilibrium hydrogen exchange for determination of H-bond strength and water accessibility in solid proteins

Inhibitors of PEX14 disrupt protein import into glycosomes and kill Trypanosoma parasites

Integrated structural biology to unravel molecular mechanisms of protein-RNA recognition

Allosteric modulation of peroxisomal membrane protein recognition by farnesylation of the peroxisomal import receptor PEX19

Focal adhesion stabilization by enhanced integrin-cRGD binding affinity

The glia of the adult Drosophila nervous system

Epigallocatechin-3-gallate preferentially induces aggregation of amyloidogenic immunoglobulin light chains

A two-component adhesive: Tau fibrils arise from a combination of a well-defined motif and conformationally flexible interactions

Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia in Drosophila

Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex

A Comprehensive Evaluation of the Activity and Selectivity Profile of Ligands for RGD-binding Integrins

The NLRP3 Inflammasome Renders Cell Death Pro-inflammatory

2016

Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions

In-vivo PET imaging of the "cancer integrin" αvβ6 using gallium-68 labelled cyclic RGD nonapeptides

Structural basis for ArfA–RF2-mediated translation termination on mRNAs lacking stop codons

Deciphering the Translation Initiation Factor 5A Modification Pathway in Halophilic Archaea

Structural basis for the recognition of spliceosomal SmN/B/B’ proteins by the RBM5 OCRE domain in splicing regulation

Recognition of the 3′ splice site RNA by the U2AF heterodimer involves a dynamic population shift

Rational Design of Cyclic Peptide Inhibitors of U2AF Homology Motif (UHM) Domains To Modulate Pre-mRNA Splicing

cGAS-Mediated Innate Immunity Spreads Intercellularly through HIV-1 Env-Induced Membrane Fusion Sites

Importance of cycle timing for the function of the molecular chaperone Hsp90

Structural biology of the import pathways of peroxisomal matrix proteins

DNA annealing by Redβ is insufficient for homologous recombination and the additional requirements involve intra- and inter-molecular interactions

Investigating the Role of Large-Scale Domain Dynamics in Protein-Protein Interactions

Site-Specific Isotope-Labeling of Inosine Phosphoramidites and NMR Analysis of an Inosine-Containing RNA Duplex

Bacterial Protein Synthesis as a Target for Antibiotic Inhibition

A combined cryo-EM and molecular dynamics approach reveals the mechanism of ErmBL-mediated translation arrest

Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts

Structures of the orthosomycin antibiotics avilamycin and evernimicin in complex with the bacterial 70S ribosome

Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length

Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding

Structural and biochemical characterization of the cell fate determining nucleotidyltransferase fold protein MAB21L1

Solution structure and binding specificity of the p63 DNA binding domain

RIG-I-Like Receptors: One STrEP Forward

The stringent factor RelA adopts an open conformation on the ribosome to stimulate ppGpp synthesis

Invited review: Architectures and mechanisms of ATP binding cassette proteins

Variation of Specific Activities of Ga-68-Aquibeprin and Ga-68-Avebetrin Enables Selective PET-Imaging of Different Expression Levels of Integrins α₅β₁ and α_vβ₃

αvβ3- or α5β1-Integrin-Selective Peptidomimetics for Surface Coating

The ABC of Ribosome-Related Antibiotic Resistance

Recognition of Endogenous Nucleic Acids by the Innate Immune System

Roquin recognizes a non-canonical hexaloop structure in the 3′-UTR of Ox40

Immobilization of soluble protein complexes in MAS solid-state NMR: Sedimentation versus viscosity

Dual-targeting triplebody 33-3-19 mediates selective lysis of biphenotypic CD19⁺ CD33⁺ leukemia cells

Novel small molecules targeting ciliary transport of Smoothened and oncogenic Hedgehog pathway activation

Pitchfork and Gprasp2 Target Smoothened to the Primary Cilium for Hedgehog Pathway Activation

Sulindac Sulfide Induces the Formation of Large Oligomeric Aggregates of the Alzheimer’s Disease Amyloid-β Peptide Which Exhibit Reduced Neurotoxicity

Structural mechanism of ATP-dependent DNA binding and DNA end bridging by eukaryotic Rad50

Translation regulation via nascent polypeptide-mediated ribosome stalling

A Stable Mutant Predisposes Antibody Domains to Amyloid Formation through Specific Non-Native Interactions

Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress

Structure of the mammalian antimicrobial peptide Bac7(1–16) bound within the exit tunnel of a bacterial ribosome

Improved Accuracy from Joint X-ray and NMR Refinement of a Protein–RNA Complex Structure

Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68

Inhibition of Canonical NF-κB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction

RNA recognition by Roquin in posttranscriptional gene regulation

2015

Structure of the hypusinylated eukaryotic translation factor eIF-5A bound to the ribosome

Stable Peptides Instead of Stapled Peptides: Highly Potent αvβ6-Selective Integrin Ligands

Small Cause, Great Impact: Modification of the Guanidine Group in the RGD Motif Controls Integrin Subtype Selectivity

Caveolin-1-negative head and neck squamous cell carcinoma primary tumors display increased epithelial to mesenchymal transition and prometastatic properties

Restoring Resolution in Biological Solid-State NMR under Conditions of Off-Magic-Angle Spinning

ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA

Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction

Blast from the Past: Reassessing ForgottenTranslation Inhibitors, Antibiotic Selectivity, and ResistanceMechanisms to Aid Drug Development

Blast from the Past: Reassessing Forgotten Translation Inhibitors, Antibiotic Selectivity, and Resistance Mechanisms to Aid Drug Development

The Antibody Light-Chain Linker Is Important for Domain Stability and Amyloid Formation

Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling

Stall no more at polyproline stretches with the translation elongation factors EF-P and IF-5A

Hsp90·Cdc37 Complexes with Protein Kinases Form Cooperatively with Multiple Distinct Interaction Sites

Structure of a Human 4E-T/DDX6/CNOT1 Complex Reveals the Different Interplay of DDX6-Binding Proteins with the CCR4-NOT Complex

In vivo biokinetic and metabolic characterization of the ⁶⁸Ga-labelled α5β1-selective peptidomimetic FR366

Bioactive compounds immobilized on Ti and TiNbHf: AFM-basedinvestigations of biofunctionalization efficiency and cell adhesion

Integrin α5β1 and p53 convergent pathways in the control of anti-apoptotic proteins PEA-15 and survivin in high-grade glioma

The chaperone αB-crystallin uses different interfaces to capture an amorphous and an amyloid client

Structural biology of the import pathways of peroxisomalmatrix proteins

Towards the molecular mechanism of the integration of peroxisomal membrane proteins

Structural Mechanism of the Interaction of Alzheimer Disease A Fibrils with the Non-steroidal Anti-inflammatory Drug (NSAID) Sulindac Sulfide

Entropic Contribution of Elongation Factor P to Proline Positioning at the Catalytic Center of the Ribosome

Translational arrest by a prokaryotic signal recognition particle is mediated by RNA interactions

cis-Peptide Bonds: A Key for Intestinal Permeability of Peptides?

Efficient segmental isotope labeling of multi-domain proteins using Sortase A

Structural basis for recognition and remodeling of the TBP:DNA:NC2 complex by Mot1

Minimal Synthetic Cells to Study Integrin-Mediated Adhesion

RNA degradation paths in a 12-subunit nuclear exosome complex

Systematic Backbone Conformational Constraints on a Cyclic Melanotropin Ligand Leads to Highly Selective Ligands for Multiple Melanocortin Receptors

Structural analysis of the diadenylate cyclase reaction of DNA-integrity scanning protein A (DisA) and its inhibition by 3-dATP

Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3

A Crystallin Fold in the Interleukin-4-inducing Principle of Schistosoma mansoni Eggs (IPSE/α-1) Mediates IgE Binding for Antigen-independent Basophil Activation

Saccharomyces cerevisiae Ski7 Is a GTP-Binding Protein Adopting the Characteristic Conformation of Active Translational GTPases

Structure of the catalytic domain of Mre11 from Chaetomium thermophilum

Deciphering the protein-RNA recognition code: Combining large-scale quantitative methods with structural biology

Distinct tRNA Accommodation Intermediates Observed on the Ribosome with the Antibiotics Hygromycin A and A201A

Selective activators of protein phosphatase 5 target the auto-inhibitory mechanism

The EBNA-2 N-Terminal Transactivation Domain Folds into a Dimeric Structure Required for Target Gene Activation

Segregation Versus Colocalization: Orthogonally Functionalized Binary Micropatterned Substrates Regulate the Molecular Distribution in Focal Adhesions

Structural and Functional Analysis of the Signal-Transducing Linker in the pH-Responsive One-Component System CadC of Escherichia coli

Architecture of the Ubiquitylation Module of the Yeast Ccr4-Not Complex

Enantiomeric Cyclic Peptides with Different Caco-2 Permeability Suggest Carrier-Mediated Transport

A novel RNA binding surface of the TAM domain of TIP5/BAZ2A mediates epigenetic regulation of rRNA genes

Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling

Structural Characterization of LRRK2 Inhibitors

Cryo-EM structure of the tetracycline resistance protein TetM in complex with a translating ribosome at 3.9-Å resolution

Bruce Merrifield Award & Murray Goodman Endowed Lectureship for CIPSM researcher Horst Kessler

Hsp90 regulates the dynamics of its cochaperone Sti1 and the transfer of Hsp70 between modules

Mimicking bone extracellular matrix: Integrin-binding peptidomimetics enhance osteoblast-like cells adhesion, proliferation, and differentiation on titanium

Chapter Eleven – Structural Analysis of Protein–RNA Complexes in Solution Using NMR Paramagnetic Relaxation Enhancements

Dynamic RNA world

Structural Basis for Dodecameric Assembly States and Conformational Plasticity of the Full-Length AAA+ ATPases Rvb1·Rvb2

Disclosing the CXCR4 Expression in Lymphoproliferative Diseases by Targeted Molecular Imaging

The proline-rich antimicrobial peptide Onc112 inhibits translation by blocking and destabilizing the initiation complex

Arginine-rhamnosylation as new strategy to activate translation elongation factor P

Cloning, expression and purification of the human Islet Amyloid Polypeptide (hIAPP) from Escherichia coli

A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease

Site-Specific Solid-State NMR Studies of “Trigger Factor” in Complex with the Large Ribosomal Subunit 50S

Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization

Direct prediction of residual dipolar couplings of small molecules in a stretched gel by stochastic molecular dynamics simulations

Access to Cα Backbone Dynamics of Biological Solids by 13C T1 Relaxation and Molecular Dynamics Simulation

2014

Breaking the protein-RNA recognition code

Molecular architecture of the HerA–NurA DNA double-strand break resection complex

Annotation of genomics data using bidirectional hidden Markov models unveils variations in Pol II transcription cycle

Detection of Hydrogen Bonds in Dynamic Regions of RNA by NMR Spectroscopy

Differential inhibition of Arabidopsis superoxide dismutases by peroxynitrite-mediated tyrosine nitration

Structure of the Rad50 DNA double-strand break repair protein in complex with DNA

A Conserved Proline Triplet in Val-tRNA Synthetase and the Origin of Elongation Factor P

Drug Sensing by the Ribosome Induces Translational Arrest via Active Site Perturbation

The bacterial translation stress response

Artificial Accelerators of the Molecular Chaperone Hsp90 Facilitate Rate-Limiting Conformational Transitions

Receptor-Bound Conformation of Cilengitide Better Represented by Its Solution-State Structure than the Solid-State Structure

ATP puts the brake on DNA double-strand break repair

Structural basis for the assembly of the Sxl–Unr translation regulatory complex

Transcriptome Maps of mRNP Biogenesis Factors Define Pre-mRNA Recognition

A Novel Protein-Protein Interaction in the RES (REtention and Splicing) Complex

Translational stalling at polyproline stretches is modulated by the sequence context upstream of the stall site

Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation

A Residue-specific Shift in Stability and Amyloidogenicity of Antibody Variable Domains

Structural Studies of DNA End Detection and Resection in Homologous Recombination

T cell-recruiting triplebody 19-3-19 mediates serial lysis of malignant B-lymphoid cells by a single T cell

OAS proteins and cGAS: unifying concepts in sensing and responding to cytosolic nucleic acids

Autophagic Clearance of PolyQ Proteins Mediated by Ubiquitin-Atg8 Adaptors of the Conserved CUET Protein Family

Structural basis for RNA recognition in roquin-mediated post-transcriptional gene regulation

Protein Folding by Interaction

NMR approaches for structural analysis of multidomain proteins and complexes in solution

Common variants in the HLA-DQ region confer susceptibility to idiopathic achalasia

Site-specific analysis of heteronuclear Overhauser effects in microcrystalline proteins

A DNA-Dependent Protease Involved in DNA-Protein Crosslink Repair

The Integrin Ligand c(RGDf(NMe)Nal) Reduces Neointimal Hyperplasia in a Polymer-Free Drug-Eluting Stent System

Structure-Based Prediction of Asparagine and Aspartate Degradation Sites in Antibody Variable Regions

Rational Improvement of the Affinity and Selectivity of Integrin Binding of Grafted Lasso Peptides

The structural analysis of shark IgNAR antibodies reveals evolutionary principles of immunoglobulins

Rpb4 Subunit Functions Mainly in mRNA Synthesis by RNA Polymerase II

Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production

Periodic mRNA synthesis and degradation co-operate during cell cycle gene expression

In Vivo Ligands of MDA5 and RIG-I in Measles Virus- Infected Cells

The dynamic duo: Combining NMR and small angle scattering in structural biology

Entrapment of DNA in an intersubunit tunnel system of a single-stranded DNA-binding protein

Transient Electrostatic Interactions Dominate the Conformational Equilibrium Sampled by Multidomain Splicing Factor U2AF65: A Combined NMR and SAXS Study

Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome

Leucine-Rich Repeat Kinase 2 Binds to Neuronal Vesicles through Protein Interactions Mediated by Its C-Terminal WD40 Domain

Benefits of NOPO As Chelator in Gallium-68 Peptides, Exemplified by Preclinical Characterization of 68Ga-NOPO−c(RGDfK

Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1

Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide

Pharmacophoric Modifications Lead to Superpotent αvβ3 Integrin Ligands with Suppressed α5β1 Activity

Modulation of the Hsp90 Chaperone Cycle by a Stringent Client Protein

Next-Generation Heteronuclear Decoupling for High-Field Biomolecular NMR Spectroscopy

Dynamics in the solid-state: perspectives for the investigation of amyloid aggregates, membrane proteins and soluble protein complexes

Ribosome Rescue, Nearing the End

Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11

Crosstalk between the cGAS DNA Sensor and Beclin-1 Autophagy Protein Shapes Innate Antimicrobial Immune Responses

A new system for naming ribosomal proteins

Investigation of early cell–surface interactions of human mesenchymal stem cells on nanopatterned β-type titanium–niobium alloy surfaces

Tetracycline antibiotics and resistance mechanisms

RNA polymerase II termination involves C-terminal-domain tyrosine dephosphorylation by CPF subunit Glc7

Ribosome-targeting antibiotics and mechanisms of bacterial resistance

A novel intermediate in transcription initiation by human mitochondrial RNA polymerase

The Cyclic Cystine Ladder of Theta-Defensins as a Stable, Bifunctional Scaffold: A Proof-of-Concept Study Using the Integrin-Binding RGD Motif.

2013

Studying Weak and Dynamic Interactions of Posttranslationally Modified Proteins using Expressed Protein Ligation

Transcriptome Surveillance by Selective Termination of Noncoding RNA Synthesis

Recruitment of TREX to the Transcription Machinery by Its Direct Binding to the Phospho-CTD of RNA Polymerase II

A Novel Pex14 Protein-interacting Site of Human Pex5 Is Critical for Matrix Protein Import into Peroxisomes

The RNA Polymerase II C-terminal Domain-Interacting Domain of Yeast Nrd1 Contributes to the Choice of Termination Pathway and Couples to RNA Processing by the Nuclear Exosome

Insights into the Mode of Action of Novel Fluoroketolides, Potent Inhibitors of Bacterial Protein Synthesis

RNA polymerase I structure and transcription regulation

Cap Completion and C-Terminal Repeat Domain Kinase Recruitment Underlie the Initiation-Elongation Transition of RNA Polymerase II

Interface Immobilization Chemistry of cRGD-based Peptides Regulates Integrin Mediated Cell Adhesion

Integrin modulators: a patent review

Global Analysis of Eukaryotic mRNA Degradation Reveals Xrn1-Dependent Buffering of Transcript Levels

Structure of human mitochondrial RNA polymerase elongation complex

Opening windows into the cell: focused-ion-beam milling for cryo-electron tomography

Selective Imaging of the Angiogenic Relevant Integrins α5β1 and αvβ3

Structures of RNA polymerase II complexes with Bye1, a chromatin-binding PHF3/DIDO homologue

Amythiamicin D and Related Thiopeptides as Inhibitors of the Bacterial Elongation Factor EF-Tu: Modification of the Amino Acid at Carbon Atom C2 of Ring C Dramatically Influences Activity

Structure and Subunit Topology of the INO80 Chromatin Remodeler and Its Nucleosome Complex

On the use of the antibiotic chloramphenicol to target polypeptide chain mimics to the ribosomal exit tunnel

Unveiling the Long-Held Secrets of the 26S Proteasome

Distinct XPPX sequence motifs induce ribosome stalling, which is rescued by the translation elongation factor EF-P

Tumor targeting via integrin ligands

The Glycophorin A Transmembrane Sequence within Integrin αvβ3 Creates a Non-Signaling Integrin with Low Basal Affinity That Is Strongly Adhesive under Force

Cryogenic solid state NMR studies of fibrils of the Alzheimer’s disease amyloid-β peptide: perspectives for DNP

Model of the Mediator middle module based on protein cross-linking

Cryo-electron tomography: The challenge of doing structural biology in situ

NMR Spectroscopy of Soluble Protein Complexes at One Mega-Dalton and Beyond

A Molecular Toolkit for the Functionalization of Titanium-Based Biomaterials That Selectively Control Integrin-Mediated Cell Adhesion

High-resolution structures of the IgM Fc domains reveal principles of its hexamer formation

Proton-Detected Solid-State NMR Spectroscopy at Aliphatic Sites: Application to Crystalline Systems

The RNA polymerase trigger loop functions in all three phases of the transcription cycle

Comparison of cyclic RGD peptides for αvβ3 integrin detection in a rat model of myocardial infarction

Crystal Structure of Human TWEAK in Complex with the Fab Fragment of a Neutralizing Antibody Reveals Insights into Receptor Binding

Hydrogen bonding involving side chain exchangeable groups stabilizes amyloid quarternary structure

Structures of the human and Drosophila 80S ribosome

Drosophila miR-277 controls branched-chain amino acid catabolism and affects lifespan

Structure of the 26S proteasome with ATP-γS bound provides insights into the mechanism of nucleotide-dependent substrate translocation

A Cyclen-Based Tetraphosphinate Chelator for the Preparation of Radiolabeled Tetrameric Bioconjugates

Species-specific detection of the antiviral small-molecule compound CMA by STING

The Mechanism of Denaturation and the Unfolded State of the α-Helical Membrane-Associated Protein Mistic

Oral Disinfectants Inhibit Protein–Protein Interactions Mediated by the Anti-Apoptotic Protein Bcl-xL and Induce Apoptosis in Human Oral Tumor Cells

Nascent peptides that block protein synthesis in bacteria

Combining NMR and small angle X-ray and neutron scattering in the structural analysis of a ternary protein-RNA complex

Struggling to let go: a non-coding RNA directs its own extension and destruction

Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis

Protein–RNA Interfaces Probed by 1H-Detected MAS Solid-State NMR Spectroscopy

Translational repression of thymidylate synthase by targeting its mRNA

Production of a de-novo designed antimicrobial peptide in Nicotiana benthamiana

Crystal structure of an RNA-bound 11-subunit eukaryotic exosome complex

Biselectivity of isoDGR Peptides for Fibronectin Binding Integrin Subtypes α5β1 and αvβ6: Conformational Control through Flanking Amino Acids

Functionalizing αvβ3- or α5β1-Selective Integrin Antagonists for Surface Coating: A Method To Discriminate Integrin Subtypes In Vitro

Structural and Functional Analysis of the DEAF-1 and BS69 MYND Domains

Paramyxovirus V Proteins Disrupt the Fold of the RNA Sensor MDA5 to Inhibit Antiviral Signaling

FOSL1 controls the assembly of endothelial cells into capillary tubes by direct repression of αv and β3 integrin transcription.

The Regulatory Domain Stabilizes the p53 Tetramer by Intersubunit Contacts with the DNA Binding Domain

2012

Structure and function of the initially transcribing RNA polymerase II–TFIIB complex

Translation Elongation Factor EF-P Alleviates Ribosome Stalling at Polyproline Stretches

Funktionalisierung αvβ3- oder α5β1-selektiver Integrinantagonisten für die Oberflächenbeschichtung: ein Hilfsmittel zur Unterscheidung von Integrinsubtypen in vitro

Promiscuous behaviour of archaeal ribosomal proteins: Implications for eukaryotic ribosome evolution

Structure, phosphorylation and U2AF65 binding of the N-terminal domain of splicing factor 1 during 30-splice site recognition

N-Methylierung von Peptiden und Proteinen: ein wichtiges Element für die Regulation biologischer Funktionen

Optimal degree of protonation for 1H detection of aliphatic sites in randomly deuterated proteins as a function of the MAS frequency

Structural basis for TetM-mediated tetracycline resistance

Structural Properties of EGCG-Induced, Nontoxic Alzheimer's Disease Aβ Oligomers

A Conformationally Frozen Peptoid Boosts CXCR4 Affinity and Anti-HIV Activity

Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avb3-selective cyclic RGD peptides

CTD Tyrosine Phosphorylation Impairs Termination Factor Recruitment to RNA Polymerase II

Intestinal Permeability of Cyclic Peptides: Common Key Backbone Motifs Identified

Determination of solution structures of proteins up to 40 kDa using CS-Rosetta with sparse NMR data from deuterated samples

Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins

A Movie of RNA Polymerase II Transcription

MC EMiNEM Maps the Interaction Landscape of the Mediator

Dynamics in multi-domain protein recognition of RNA

Structure of Mre11–Nbs1 complex yields insights into ataxia-telangiectasia–like disease mutations and DNA damage signaling

Lys34 of translation elongation factor EF-P is hydroxylated by YfcM

EMBO welcomes 3 CIPSM scientists as new members

An Asymmetric Dimer as the Basic Subunit in Alzheimer’s Disease Amyloid β Fibrils

Swi2/Snf2 remodelers: hybrid views on hybrid molecular machines

Mechanism of Translesion Transcription by RNA Polymerase II and Its Role in Cellular Resistance to DNA Damage

Structural and Functional Organization of the Ska Complex, a Key Component of the Kinetochore-Microtubule Interface

Comparative Dynamic Transcriptome Analysis (cDTA) reveals mutual feedback between mRNA synthesis and degradation

Substrate Transport Activation Is Mediated through Second Periplasmic Loop of Transmembrane Protein MalF in Maltose Transport Complex of Escherichia coli

Dynamic Architecture of a Minimal RNA Polymerase II Open Promoter Complex

Crosslinking-MS analysis reveals RNA polymerase I domain architecture and basis of rRNA cleavage

Radiolabelled RGD peptides for imaging and therapy

Structural basis of highly conserved ribosome recycling in eukaryotes and archaea

Solid-phase-assisted synthesis of targeting peptide–PEG–oligo(ethane amino)amides for receptor-mediated gene delivery

Conservation between the RNA Polymerase I, II, and III Transcription Initiation Machineries

Target- and Resistance-Based Mechanistic Studies with TP-434, a Novel Fluorocycline Antibiotic

A structural perspective on Mediator function

hnRNP A1 Proofreads 30 Splice Site Recognition by U2AF

Synthesis of N-methylated cyclic peptides

Processive RNA decay by the exosome

Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy without Purification and Reconstitution

Synthesis and Conformational Analysis of Efrapeptins

Chromodomains read the arginine code of post-translational targeting

The architecture of functional modules in the Hsp90 co-chaperone Sti1/Hop

Structural and Mechanistic Implications of Metal-Binding in the Small Heat-Shock Protein αB-Crystallin

Solution Structure and Molecular Interactions of Lamin B Receptor Tudor Domain

Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: Implications for structure and dynamics

Sensing of viral nucleic acids by RIG-I: From translocation to translation

2011

On the specificity of antibiotics targeting the large ribosomal subunit

Tailoring of Integrin Ligands: Probing the Charge Capability of the Metal Ion-Dependent Adhesion Site

Spectroscopically Well-Characterized RGD Optical Probe as a Prerequisite for Lifetime-Gated Tumor Imaging

Assignment strategies for aliphatic protons in the solid-state in randomly protonated proteins

Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins

The DARC site: a database of aligned ribosomal complexes

The RIG-I ATPase domain structure reveals insights into ATP-dependent antiviral signalling

Increasing αvβ3 Selectivity of the Anti-Angiogenic Drug Cilengitide by N-Methylation

Increasing alpha-v-beta-3 Selectivity of the Anti-Angiogenic Drug Cilengitide by N-Methylation

Structure of human mitochondrial RNA polymerase

Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth

ATP driven structural changes of the bacterial Mre11:Rad50 catalytic head complex

A Glycopeptide Dendrimer Inhibitor of the Galactose-Specific Lectin LecA and of Pseudomonas aeruginosa Biofilms

Design, Synthesis, and Functionalization of Dimeric Peptides Targeting Chemokine Receptor CXCR4

Introducing Lasso Peptides as Molecular Scaffolds for Drug Design: Engineering of an Integrin Antagonist

Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53

Nuclear actin-related proteins take shape

Strategies for the structural analysis of multi-protein complexes: Lessons from the 3D-Repertoire project

Telethonin Deficiency Is Associated With Maladaptation to Biomechanical Stress in the Mammalian Heart

1H, 13C, 15N and 31P chemical shift assignments of a human Xist RNA A-repeat tetraloop hairpin essential for X-chromosome inactivation

PET Imaging of CXCR4 Receptors in Cancer by a New Optimized Ligand

How to make stripes: deciphering the transition from non-periodic to periodic patterns in Drosophila segmentation

Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF

Structure and mechanism of the Swi2/Snf2 remodeller Mot1 in complex with its substrate TBP

Evolution of Two Modes of Intrinsic RNA Polymerase Transcript Cleavage

Differential Effects of Thiopeptide and Orthosomycin Antibiotics on Translational GTPases

Optimal 2H rf Pulses and 2H–13C Cross-Polarization Methods for Solid-State 2H MAS NMR of Perdeuterated Proteins

A Conformational Switch Underlies ClpP Protease Function

Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins

1H, 13C and 15N chemical shift assignments of IPSEΔNLS

Iwr1 Directs RNA Polymerase II Nuclear Import

The Effect of Multiple N-Methylation on Intestinal Permeability of Cyclic Hexapeptides

Cryo-EM structure of the ribosome–SecYE complex in the membrane environment

Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization

Structural biochemistry of nuclear actin-related proteins 4 and 8 reveals their interaction with actin

N-Methylated sst2 Selective Somatostatin Cyclic Peptide Analogue as a Potent Candidate for Treating Neurogenic Inflammation

A Cytoplasmic Complex Mediates Specific mRNA Recognition and Localization in Yeast

The Mre11:Rad50 Structure Shows an ATP-Dependent Molecular Clamp in DNA Double-Strand Break Repair

Structural Analysis of Large Protein Complexes Using Solvent Paramagnetic Relaxation Enhancements

Structure Calculation from Unambiguous Long-Range Amide and Methyl 1H−1H Distance Restraints for a Microcrystalline Protein with MAS Solid-State NMR Spectroscopy

Take a look at Karl-Peter Hopfner's "Sensing DNA Damage" Cell Movie

NMR and small-angle scattering-based structural analysis of protein complexes in solution

Architecture of the RNA polymerase–Spt4/5 complex and basis of universal transcription processivity

Structure and VP16 binding of the Mediator Med25 activator interaction domain

Structural basis of RNA polymerase II backtracking, arrest and reactivation

Bacterial Inclusion Bodies of Alzheimer’s Disease b-Amyloid Peptides Can Be Employed To Study Native-Like Aggregation Intermediate States

The ribosomal tunnel as a functional environment for nascent polypeptide folding and translational stalling

Peptides in the Ribosomal Tunnel Talk Back

SecM-Stalled Ribosomes Adopt an Altered Geometry at the Peptidyl Transferase Center

PET Imaging of Integrin alpha-V-beta-3 Expression

Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast

pH-Dependent Dimerization and Salt-Dependent Stabilization of the N-terminal Domain of Spider Dragline Silk-Implications for Fiber Formation

Stable functionalized PEGylated quantum dots micelles with a controlled stoichiometry

Proteomic Characterization of Archaeal Ribosomes Reveals the Presence of Novel Archaeal-Specific Ribosomal Proteins

2010

Architecture of the RNA polymerase II–TFIIF complex revealed by cross-linking and mass spectrometry

Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites

Structure and DNA-binding activity of the Pyrococcus furiosus SMC protein hinge domain

CIPSM's Daniel Wilson received the EMBO Young Investigators Award for his work on new antibiotics

RNA polymerase II–TFIIB structure and mechanism of transcription initiation

Reversible and Controllable Nanolocomotion of an RNA-Processing Machinery

Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution

Mechanism of replication blocking and bypass of Y-family polymerase Eta by bulky acetylaminofluorene DNA adducts

The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress

NCO-sP(EO-stat-PO) surface coatings preserve biochemical properties of RGD peptides

NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1

Optimized Measurement Temperature Gives Access to the Solution Structure of a 49 kDa Homohexameric Beta - Propeller

Structural Basis for Translational Stalling by Human Cytomegalovirus and Fungal Arginine Attenuator Peptide

A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II CTD

Molecular Basis of RNA Polymerase III Transcription Repression by Maf1

Polymeric Substrates with Tunable Elasticity and Nanoscopically Controlled Biomolecule Presentation

Uniform transitions of the general RNA polymerase II transcription complex

Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function

RNA Polymerase I Contains a TFIIF-Related DNA-Binding Subcomplex

Structural Basis for Adenylate Kinase Activity in ABC ATPases

Structure of an atypical Tudor domain in the Drosophila Polycomblike protein

Improvement of drug-like properties of peptides: the somatostatin paradigm

Structural Basis for Homodimerization of the Src-associated during Mitosis, 68-kDa Protein (Sam68) Qua1 Domain

Probing Translation with Small-Molecule Inhibitors

The archaeo-eukaryotic primase of plasmid pRN1 requires a helix bundle domain for faithful primer synthesis

Interplay between the Ribosomal Tunnel, Nascent Chain, and Macrolides Influences Drug Inhibition

Nucleocytoplasmic shuttling of the La motif-containing protein Sro9 might link its nuclear and cytoplasmic functions

Towards molecular systems biology of gene transcription and regulation

Multiple N-Methylation of MT-II Backbone Amide Bonds Leads to Melanocortin Receptor Subtype hMC1R Selectivity: Pharmacological and Conformational Studies

A conserved spider silk domain acts as a molecular switch that controls fibre assembly

Structural Analysis of Protein Interfaces from 13C Direct-Detected Paramagnetic Relaxation Enhancements

Cilengitide: The First Anti-Angiogenic Small Molecule Drug Candidate. Design, Synthesis and Clinical Evaluation

Structure and RNA recognition by the snRNA and snoRNA transport factor PHAX

Quantitative analysis of processive RNA degradation by the archaeal RNA exosome

The Impact of Amino Acid Side Chain Mutations in Conformational Design of Peptides and Proteins

Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif

Cell Adhesion Strength Is Controlled by Intermolecular Spacing of Adhesion Receptors

Asymmetric Activation of the Hsp90 Dimer by Its Cochaperone Aha1

An Efficient Protocol for NMR-Spectroscopy-Based Structure Determination of Protein Complexes in Solution

α-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel

Structure and DNA binding activity of the mouse condensin hinge domain highlight common and diverse features of SMC proteins

Preparation and topology of the Mediator middle module

BclxL Changes Conformation upon Binding to Wild-type but Not Mutant p53 DNA Binding Domain

Coupled chaperone action in folding and assembly of hexadecameric Rubisco

Imaging of integrin avb3 expression in patients with malignant glioma by [18F] Galacto-RGD positron emission tomography

The Transmembrane Structure of Integrin aIIbb3: Significance for Signal Transduction

Force-induced destabilization of focal adhesions at defined integrin spacings on nanostructured surfaces

2009

Modulation of protein properties in living cells using nanobodies

PSRP1 Is Not a Ribosomal Protein, but a Ribosome-binding Factor That Is Recycled by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G)

Structural Insight into Nascent Polypeptide Chain–Mediated Translational Stalling

Structure of Monomeric Yeast and Mammalian Sec61 Complexes Interacting with the Translating Ribosome

The A–Z of bacterial translation inhibitors

Biosynthesis of the Aminocyclitol Subunit of Hygromycin A in Streptomyces hygroscopicus NRRL 2388

Identification of Distinct Thiopeptide-Antibiotic Precursor Lead Compounds Using Translation Machinery Assays

X-ray structure of Pur-α reveals a Whirly-like fold and an unusual nucleic-acid binding surface

Lessons from structural and biochemical studies on the archaeal exosome

Enhanced SnapShot: Antibiotic Inhibition of Protein Synthesis II

DNA Double-Strand Breaks Come into Focus

A Boost for Sequence Searching

SnapShot: Antibiotic Inhibition of Protein Synthesis I

The torpedo nuclease Rat1 is insufficient to terminate RNA polymerase II in vitro

Molecular basis of transcriptional mutagenesis at 8-oxoguanine

The Final Step of Hygromycin A Biosynthesis, Oxidation of C-5''-Dihydrohygromycin A, Is Linked to a Putative Proton Gradient-Dependent Efflux

Formation of She2p tetramers is required for mRNA binding, mRNP assembly, and localization

Hsp90 is regulated by a switch point in the C-terminal domain

Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences

Prolonged stability by cyclization: Macrocyclic phosphino dipeptide isostere inhibitors of b-secretase (BACE1)

Helical Growth of the Arabidopsis Mutant tortifolia2 Does Not Depend on Cell Division Patterns but Involves Handed Twisting of Isolated Cells

mRNA Export - An Integrative Component of Gene Expression

Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complex

The Transmembrane Structure of Integrin [alpha]IIb[beta]3: Significance for Signal Transduction

Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II

Structural Basis of Transcription: Mismatch-Specific Fidelity Mechanisms and Paused RNA Polymerase II with Frayed RNA

Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2.

Fast and accurate automatic structure prediction with HHpred

An Unfolded CH1 Domain Controls the Assembly and Secretion of IgG Antibodies

Adhesion Dance with Raver

Breaking the Dogma of the Metal-Coordinating Carboxylate Group in Integrin Ligands: Introducing Hydroxamic Acids to the MIDAS To Tune Potency and Selectivity

Ligands for Mapping αvβ3-Integrin Expression in Vivo

A movie of the RNA polymerase nucleotide addition cycle

Structure and in Vivo Requirement of the Yeast Spt6 SH2 Domain

HHomp—prediction and classification of outer membrane proteins

Non-Hydrolyzable RNA-Peptide Conjugates: A Powerful Advance in the Synthesis of Mimics for 3-Peptidyl tRNA Termini

The HSP90 binding mode of a radicicol-like E-oxime determined by docking, binding free energy estimations, and NMR 15N chemical shifts

Interaction of human heat shock protein 70 with tumor-associated peptides

Sequence context-specific profiles for homology searching

Cytosolic Viral Sensor RIG-I Is a 5′-Triphosphate–Dependent Translocase on Double-Stranded RNA

Structure and Ligand Binding of the Extended Tudor Domain of D. melanogaster Tudor-SN

Impact of Order and Disorder in RGD Nanopatterns on Cell Adhesion

The regulatory domain of the RIG-I family ATPase LGP2 senses double-stranded RNA